Tyne River Macroinvertebrates 2020
Newcastle University
Description
Macroinvertebrates collected by kick sample from the River Tyne at NZ 107 645 on 6 November 2020 by John Bythell
Sequence data to be submitted to BOLD.
Geographic Description
Kick sample collection, River Tyne near Wylam
Purpose
Opportunistic sampling to research molecular barcoding applications.
Data quality
Submissions here only include samples that were reliably identified to species level by the molecular barcode (>99% identification reliability on BOLD https://v4.boldsystems.org/) Molecular ID was only deemed reliable if consistent with UKSI-listed species.
Methods
Representative individuals of a combined kick-sample of invertebrates were sorted, photographed and individually dissected to collect tissue samples for molecular barcoding using cytochrome oxidase subunit 1 (CO1) gene amplification using standard Folmer primers (Folmer et al 1994 Mol Mar Biol Biotechnol. 3(5):294-9) and BF2/BR2 primers ( Elbrecht & Leese (2017) Front. Environ. Sci., https://doi.org/10.3389/fenvs.2017.00011). Sequencing was carried out in both directions to create consensus assembly sequence up to 658 bp.
Citation
Bythell JC (2021) Tyne River Macroinvertebrates 2020, Newcastle University UK.
Rights
© John Bythell, Newcastle University
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Digitised records
Looking up... the number of records that can be accessed through the NBN Atlas. This resource was last checked for updated data on 08 Jun 2021. The most recent data was published on 08 Jun 2021.
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